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Regulatory
Part:BBa_K3844001:Design
Designed by: Anya Sabhnani Group: iGEM21_DNHS_SanDiego_CA (2021-10-21)
Transcription factor: CTCF >chromosome:GRCh38:16:67561867:67639777:1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 352
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 425
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.
Source
DNA binding sites